Figure 4

Pre-treatment with rCCL2 inhibits IL-1β induction of IL-6. Wild-type astrocytes were firstly pre-incubated with rCCL2 (250 ng/ml) or media only for 12 h before the addition of IL-1β (10 or 50 ng/ml) for a further 8 h. IL-6 protein secretion in supernatant was detected by ELISA (a) and mRNA levels were measured in cell lysates by qPCR (b). IL-6 protein production increased in response to IL-1β stimulation (# p < 0.01; 2-way ANOVA). Pre-treatment with rCCL2 effectively reduced IL-6 production induced by IL-1β at 10 ng/ml (** p < 0.01). IL-6 mRNA was similarly increased after incubation with IL-1β (## p < 0.001; 2-way ANOVA). This response was reduced by pre-treatment with rCCL2 (* p < 0.05). Data represent mean + sem of three separate experiments assayed in triplicate.