Figure 11

Leukocytes in spinal ganglia from diseased birds consist of different subpopulations. For flow cytometric analysis, single cell suspensions from spinal ganglia of diseased birds were stained with a Pan-Leukocyte marker (CD45) and antibodies for T cells (CD3), cytotoxic T cells (CD8), T helper cells (CD4), T cell receptor types TCR1, TCR2 and TCR3 (TCRγδ, TCRα/vβ1, TCRα/vβ2), B cells (AV20), macrophages (Kul01) and MHC class II (2G11). (A) Gating was first performed on cells with leukocyte scatter characteristics, secondly on CD45+ cells before the proportion of different lymphocyte subpopulations of all CD45+ leukocytes was determined. (B) Data expressed as mean ± SD for different subpopulations for five birds analysed.