Protection of human neuroblastoma cell line, SK-N-SH, toxicity as assayed by FACS analysis. FACS analysis of TUNEL-positive cells in (A) mock and (B) WNV (MOI-1)-infected SK-N-SH cells treated with (C) anti-IL-1β or (D) anti-TNF-α at day 2 after infection. (E) DNase I-treated cells were used as positive control. The images depicted in panels A to E are representative data of three independent experiments. (F) TUNEL-positive cells reduced significantly in the presence of neutralizing antibodies against IL-1β and TNF-α. Data in panel F are expressed as mean ± SD for three independent experiments conducted in duplicate. **p < 0.001 as compared to mock, *p < 0.05 compared to corresponding infected cells.