Table 1 Primer sequences used for qRT-PCR
Gene | Primer Sequence (5'-3') | Amplicon | |
|---|---|---|---|
[GenBank No.] |  | (bp) | Tm (°C) |
IL-1β |  |  |  |
[GenBank:NM_000576] | Â | Â | Â |
   Forward | AGCACCTTCTTTCCCTTCATC | 86 | 56 |
   Reverse | GGACCAGACATCACCAAGC |  |  |
IL-6 | Â | Â | Â |
[GenBank:NM_000600] | Â | Â | Â |
   Forward | CCAGGAGCCCAGCTATGAAC | 84 | 57 |
   Reverse | CCCAGGGAGAAGGCAACTG |  |  |
IL-8 | Â | Â | Â |
[GenBank:NM_000584] | Â | Â | Â |
   Forward | GAACTGAGAGTGATTGAGAGTGGA | 91 | 55 |
   Reverse | CTCTTCAAAAACTTCTCCACAACC |  |  |
TNF-α |  |  |  |
[GenBank:NM_000594] | Â | Â | Â |
   Forward | CCTGCCCCAATCCCTTTATT | 81 | 55 |
   Reverse | CCCTAAGCCCCCAATTCTCT |  |  |