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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Characterization of human platelet binding of recombinant T cell receptor ligand

Figure 2

Characterization of RTL551-mouse platelet binding. A) Immobilized mouse platelets (2 × 107/ml) were incubated with fluorescently-labeled RTL551 (RTL551-Alexa488, 20 μg/ml) for 30 min at 37°C. In selected experiments, mouse platelets were stimulated with thrombin (1 U/ml). Cells were imaged using differential interference contrast (DIC) and fluorescent microscopy. B) Washed mouse platelets (2 × 107/ml) were pipetted onto surfaces coated with RTL551 (50 μg/ml) or fibrinogen (FG; 50 μg/ml) for 45 min at 37°C.

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