Figure 5

Microglia/macrophages with neurons, astrocytes, and Schwann cells. Dual immunofluorescence of microglia in the ventral horn and macrophages in the ventral nerve root of hmSOD1 rat. (A,B) GFAP-labeled astrocytes (green) and CD11b-labeled microglia (red) in a ventral horn at pre-clinical stage (A) and after clinical onset (B). Scale bar in panel A represents 100 μm for A and B. (C) Composite image showing Iba1-labeled microglia (red) near NeuN-labeled motor neurons (green) in a pre-clinical rat. Scale bar in panel C represents 200 μm. (D,E) CD11b-labeled macrophages (red) relative to βIII-tubulin-labeled axons (D, green) or S100-labeled Schwann cells in ventral nerve root after clinical onset (E, green). Scale bar in D represents 100 μm for D and E.