Figure 5
Ca2+/CaMKII-dependent LTA-mediated activation of JNK and c-Jun in RBA-1 cells. (A) Cells were pretreated with BAPTA (upper part) or TG (lower part) for 1 h and then incubated with LTA for the indicated time intervals. Cell lysates were assayed by western blot using anti-phospho-CaMKII antibody. (B) Cells were pretreated CaMI or KN-62 for 1 h and then incubated with LTA for the indicated time intervals. Cell lysates were assayed by western blot using an anti-phospho-JNK or phospho-c-Jun antibody. The membranes were stripped and re-probed with anti-GAPDH antibody as a control. Data are expressed as mean of three independent experiments (n = 3). *P < 0.05; #P < 0.01, as compared with the cells exposed to LTA alone. The figure represents one of at least three individual experiments.