Figure 6

LTA induces MMP-9 expression via Ca²⁺/CaMKII-dependent transactivation of PDGFR in RBA-1 cells. (A) Cells were pretreated with AG1296 (10 μM) or LY294002 (30 μM) for 1 h and then incubated with 50 mg/ml LTA for the indicated time intervals. (B, C) Cells were pretreated with TLR2 neutralizing antibody (TLR2 nAb), BAPTA, TG, CaMI, or KN-62 for 1 h and then incubated with 50 mg/ml LTA for the indicated time intervals. Cell lysates were subjected to SDS-PAGE and blotted using an anti-phospho-JNK or anti-phospho-c-Jun (A) and anti-phospho-PDGFR (B, C) or anti-GAPDH (as an internal control) antibody. Data are expressed as mean ± SEM (B, C) or mean (A) of three independent experiments (n = 3). *P < 0.05; ^#P < 0.01, as compared with the cells exposed to LTA alone. The figure represents one of at least three individual experiments.