Figure 6
Effects of CYT and quercetin on LPS-induced caspase-1 activation. PBMCs (2 × 106/well) were treated with CYT (1 mg/ml) or quercetin (0.1 mM) for 2 h and then stimulated with LPS (10 ng/ml) for 24 h. Caspase-1 activity was determined by a colorimetric kit using substrates (A). Caspase-1 was determined by western blot analysis (B). Data shown are the inhibition rate ± S.D. for 10 donors, measured in duplicate. *P < 0.05, significantly different from the unstimulated cells. **P < 0.05 compared to LPS alone. Quer, quercetin.