Figure 3From: Induction of heme oxygenase-1 attenuates lipopolysaccharide-induced cyclooxygenase-2 expression in mouse brain endothelial cellsLPS-induced COX-2 expression is attenuated by pretreatment with CoPP in bEnd.3 cells. A: Cells were incubated with various concentrations of CoPP for 24 h. Expression of HO-1 was determined by western blot. *P < 0.05; #P < 0.01 as compared the cells incubated with vehicle alone. B: Cells were pretreated with vehicle or ZnPP (0.1 μM) for 1 h and then incubated with CoPP (0.3 μM) for 24 h. HO-1 enzyme activity was determined. #P < 0.01 as compared within groups. C: Cells were pretreated with various concentrations of CoPP for 24 h and then incubated with LPS (100 mg/ml) for 24 h. The expression of COX-2 was determined by western blot. *P < 0.05; #P < 0.01, as compared with the cells incubated with LPS alone. D: Cells were pretreated with CoPP (0.3 μM) for 24 h and then stimulated with LPS (100 mg/ml) for the indicated time intervals. The phosphorylation of NF-κB p65 was determined by western blot. Data are summarized and expressed as mean ± SEM of four individual experiments. *P < 0.05; #P < 0.01, as compared within groups. NS: not significant.Back to article page