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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Induction of heme oxygenase-1 attenuates lipopolysaccharide-induced cyclooxygenase-2 expression in mouse brain endothelial cells

Figure 4

Over-expression of HO-1 attenuates LPS-induced COX-2 expression and PGE 2 synthesis in bEnd.3 cells. A: Cells were pretreated with or without ZnPP for 1 h, incubated with CoPP for 24 h, and then incubated with LPS (100 mg/ml) for another 24 h. The media were used to determine the levels of PGE2. The expression of COX-2 and HO-1 were determined by western blot. B: Cells were pretreated with or without hemoglobin (Hb) for 1 h, infected with adenovirus (MOI = 10) or recombinant adenovirus carrying the human HO-1 gene treatment (Adv-HO-1, MOI = 10) for 48 h, and then incubated with LPS (100 mg/ml) for another 24 h. The levels of COX-2 and HO-1 expression were determined by western blot. Data are summarized and expressed as mean ± SEM for four individual experiments. *P < 0.05; #P < 0.01 as compared within groups.

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