Chemical UL-induced changes of MnSOD-Ir cells in the MVN and IVN. (A) Illustration of a typical labeling in a representative sham-operated rat and in a chemical UL animal examined 3 days after the lesion, showing an increased number of MnSOD-Ir cells in both VN. Scare bar: 100 μm. (B) Quantitative analysis of the effects of chemical UL on MnSOD-Ir cells in the MVN and IVN. Data are mean values (± SEM) of the number of MnSOD-Ir cells in the MVN and IVN for rats that had chemical UL and observed at 4, 8 h, 1, 3, 7, and 15 days after the lesion. Data of all the postoperative times were pooled in sham animals, and mean value is shown as a horizontal line and standard error of the mean (± SEM) as two dotted lines. According to Bonferroni's principle, P < 0.0083 was considered significant. * P < 0.0083, ** P < 0.00083 versus sham values.