Figure 6

Foxp3 immunofluorescent staining in brain after 3 hrs of reperfusion. Photographs show Foxp3 staining in striatum (a) and cortex (b) regions of brain in all three groups (Sham, Vehicle and H-100). Green staining represents Foxp3-positive cells, and DAPI (which stains the nucleus) is in blue. A merged image of DAPI and Foxp3 to indicate cell-specific staining for each group is also shown. Images were taken at 200 × magnification. Scale bar = 80 μm. Graphical representation of Foxp3-positive cell counts in striatum (c) and cortex (d) regions, indicated as sham-normalized fold change are also shown; "a" for p ≤ 0.05 when compared to sham group; "b" for p ≤ 0.05 when compared to vehicle group.