Immunofluorescent staining for Bcl-xL in and around the penumbra of ipsilateral brain after 24 hrs of reperfusion. Representative images of Bcl-xL-positive cells (FITC - green fluorescence) along with nuclear staining (DAPI - blue) in Sham, Vehicle and H-100 groups. A merged image of DAPI and Bcl-xL to indicate cell specific staining for each group is also shown. Images were taken at 200 × magnification. Scale bar = 80 μm. A graphical representation of Bcl-xL-positive cell counts, indicated as sham-normalized fold change is also shown; "a" for p ≤ 0.05 when compared to sham group; "b" for p ≤ 0.05 when compared to vehicle group.