Table 1 Details of primers (forward & reverse) and probes for different genes used for real -time PCR analysis.
From: Immunomodulatory effect of Hawthorn extract in an experimental stroke model
Gene | Primer/Probe | Sequences |
|---|---|---|
IL-1β | Primer (forward) | 5'-CAC CTC TCA AGC AGA GCA CAG-3' |
| Â | Primer (reverse) | 5'-GGG TTC CAT GGT GAA GTC AAC-3' |
| Â | Probe (antisense) | 5'-TGT CCC GAC CAT TGC TGT TTC CTA GG-3' |
TNF-α | Primer (forward) | 5'-CCA GGA GAA AGT CAG CCT CCT-3' |
| Â | Primer (reverse) | 5'-TCA TAC CAG GGC TTG AGC TCA-3' |
| Â | Probe (antisense) | 5'-AGA GCC CTT GCC CTA AGG ACA CCC CT-3' |
IL-6 | Primer (forward) | 5'-CGAAAGTCAACTCCATCTGCC-3' |
| Â | Primer (reverse) | 5'-GGCAACTGGCTGGAAGTCTCT-3' |
| Â | Probe (sense) | 5'-TCAGGAACAGCTATGAAGTTTCTCTCCG-3' |
ICAM-1 | Primer (forward) | 5'-AAACGGGAGATGAATGGTACCTAC-3' |
| Â | Primer (reverse) | 5'-TGCACGTCCCTGGTGATACTC-3' |
| Â | Probe (sense) | 5'-TGCCGTGCCTTTAGCTCCCGTG-3' |
rpL32 | Primer (forward) | 5'-TGT CCT CTA AGA ACC GAA AAG CC-3' |
| Â | Primer (reverse) | 5'-CGT TGG GAT TGG TGA CTC TGA-3' |
| Â | Probe (sense) | 5'-TCG TAG AAA GAG CAG CAC AGC TGG CC-3' |