Figure 4

GSK-3β inactivation inhibits NF-κB acetylation at Lys310 but not phosphorylation. BV-2 cells (A) or primary microglia (B and C) were pretreated with vehicle or TWS119 (2.5 μM) for 30 min and then stimulated with LPS (100 ng/ml) for the indicated times. Whole cell lysates were prepared and subjected to western blotting using antibodies specific for phosphorylated (Ser276, 468 and 536), acetylated (Lys310) or total forms of p65. The immunoblots are representative of three independent experiments. Data are presented as mean ± SEM for three independent experiments. **p < 0.01 compared with respective cultures treated with LPS alone.