Rapamycin reduces mTOR phosphorylation induced by TII. (A) Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for β-actin (lower gel). (B) Quantitation of densitometry wherein p-mTOR values are reflected relative to those for β-actin. Data are representative of two different experiments. Results were analyzed by one-way ANOVA followed by Bonferroni's post-test. *P < 0.05 vs. Controls; °P < 0.05 vs. TII.