Effects of the mTOR inhibitor on NO production in activated astrocytes. Astrocytes were activated for 48 h with TII [Figure 2A] or 24 h with LI [Figure 2B]. The mTOR inhibitor, rapamycin was added to the cells in nM concentrations at the beginning of the experiments. NO production was assessed indirectly by measurement of nitrite accumulation in the incubation medium (Griess method). Data are expressed as means ± SEM (n = 6). Data are representative of 3 different experiments. Results were analyzed by one-way ANOVA followed by Bonferroni's post-test. ***P < 0.001 vs. Controls.