Effects of the mTOR inhibitor RAPA on iNOS expression elicited by LI. Total cytosolic RNA was prepared from Control, or astrocyte cells treated LI and rapamycin for different times, and used for Q-PCR analysis of iNOS expression. Data are expressed as fold change vs. each respective Control, taken as calibrator for comparative quantitation analysis of mRNA levels. Astrocytes were treated for 4 h (A) or 24 h (B). Each sample was measured in triplicate, the experiment was repeated 2 times with similar results. Where indicated, 10 nM Rapamycin was added at the beginning of the experiment. LI significantly induced iNOS expression in comparison to Controls and rapamycin increases in 4 h the iNOS mRNA expression elicited by LI. Data are means ± SEM (n = 3). §§P < 0.01 vs. LI *P < 0.05 vs. Controls; one-way ANOVA followed by Bonferroni's post-test.