Rapamycin increases the iNOS promoter in transfected C6 cells. C6 cells were stable transfected with a 2,168-bp fragment of the rat iNOS promoter. (A) The cells were treated with TII or with LPS/IFNγ for indicated time point and after varying the incubation times the luciferase activity was measured. (B) Cells were incubated with TII alone or with TII/1 nM Rapamycin for 8 h. Data are presented as the percentage of luciferase activity measured in the presence of TII and rapamycin relative to the activity of TII (taken as 100%). Data are means ± SEM of n = 4 separate cell cultures; results were analyzed by unpaired t-test. ***p < 0.01 vs. TII.