The mTOR kinase blocking increases the rate of iNOS mRNA degradation. Astrocytes were preincubated with TII alone or with TII plus 10 nM rapamycin for 6 h. After the pretreatment, actinomycin D (4 μg/ml) was added for different periods of time, as indicated. Cells were harvested at designated time points, total RNA was extracted and the iNOS expression was measured by real time PCR. The mRNA level at 0 time point (before the treatment with actinomycin D) was considered as 100%. Data are representative of 3 independent experiments. Data are means ± SEM (n = 3). ***P < 0.05 vs. TII/Rapa 10 nM; one-way ANOVA followed by Bonferroni's post-test.