Regulation of the expression of RNA-binding proteins by rapamycin in astrocytes. Total cytosolic RNA was prepared from Control, or astrocyte cells treated TII and rapamycin for different times, and used for Q-PCR analysis of iNOS expression. Data are expressed as fold change vs. each respective Control, taken as calibrator for comparative quantitation analysis of mRNA levels. Astrocytes were treated for 6-12-24 h and TTP (A), KSRP (B) and HuR (C) were assessed. Each sample was measured in triplicate, the experiment was repeated 2 times with similar results. Where indicated, 10 nM Rapamycin was added at the beginning of the experiment. Data are means ± SEM (n = 3). §§P < 0.01 vs. TII **P < 0.01 or ***P < 0.001 vs. Controls; two-way ANOVA followed by Bonferroni's post-test.