Figure 3
From: Evidence for regulatory diversity and auto-regulation at the TAC1 locus in sensory neurones
Capsaicin can induce the expression of the NK1 receptor in DRG neurones that also express SP. A. fluorescent immunohistochemical analysis of 10 μm sections from mouse neonate DRG explants with (iii and iv) anti-NK1 and (v and vi) anti-SP antibody (i, iii, v) before and (ii, iv, vi) after 24 hours incubation with 10 μM capsaicin. Ai and ii represent merged images showing cellular co-localisation of NK1 and SP (yellow). B, graphical analysis of the size distribution (in microns) of neurones within mouse neonate DRG explants showing the proportion of cells expressing both SP and NK1 expression before and after capsaicin treatment demonstrating a shift in the proportion of larger diameter cells expressing SP and the receptor (n = 3, No cells counted/measured = 242, *; p < 0.05).