Figure 5

Induction of TAC1prom by LPS is independent of ECR2. A; immunohistochemical analysis of the expression of (i) TLR4 and (ii) SP and in mouse neonate DRG. iv; merged images and cells co-expressing SP and TRL4 are highlighted in yellow (white arrows). Scale bar = 23 microns. B; bar graph demonstrating the proportion of primary DRG neurones that express βgal (as assayed using X-gal) following their transfection with each of the constructs shown in figure 2A and cultured in the absence or presence of LPS (n > 3). Proportions are adjusted relative to a control plasmid containing the CMV promoter that was transfected at the same time to normalise transfection efficiencies. C; statistical analysis of data from the previous graph demonstrating that average induction rates for TAC1prom-LacZ by LPS is not significantly affected by the presence of ECR2. D; graphical analysis of the size distribution (in microns) of neurones within ECR2-TAC1prom-LacZ transgenic DRG explants analysing transgene expression following culture in the absence or presence of LPS (n = 3) demonstrating a lack of a shift in the proportion of larger diameter cells expressing βgal (n = 3, no. βgal cells counted and measured = 170).