Figure 8

GTS has no direct effect on LAT-1-mediated kynurenine transport across the BBB and IDO-mediated kynurenine production. rBE4 cells were used as an in vitro model to assess the effects of GTS on KYN transport. A549 cells, stimulated with hIFN-γ, were used as a model to determine GTS effects on IDO activity. Figure.8A shows that GTS has no effect on LAT-1-mediated kynurenine uptake in rBE4 cells (n = 3). Figure.8B reveals that GTS has no effect on IDO-mediated kynurenine production in A549 cells based on an IDO inhibition assay (n = 3). The data are expressed as mean ± SEM. For statistical significance, ## p < 0.01 compared with the no treated A549 cells, **p < 0.01 compared with the kynurenine (40 μM) loaded rBE4 cells or hIFN-γ treated A549 cells.