Skip to main content
Figure 5 | Journal of Neuroinflammation

Figure 5

From: Reduction of neutrophil activity decreases early microvascular injury after subarachnoid haemorrhage

Figure 5

Pharmacological reduction of Neutrophils and their activity. A: Neutrophil staining in representative brain sections from untreated or vinblastine, anti PMN or PDTC treated animals sacrificed 1 hour after SAH. Note that fewer neutrophils are present in vinblastine and anti PMN treated animals and a large number are present in PDTC treated brains. B: Number of neutrophils in whole coronal brain sections. Values are % of untreated SAH animals. Neutrophils are decreased by vinblastin and anti-PMN treated and increased by PDTC treatment. C: RECA-1 (filled bars) and collagen IV (open bars) immunostaining following SAH. Values are area fractions in SAH animals as % of area fractions in sham-operated animals; both paramaters show trend or significant improvements in treated animals. D: Effect of PDTC treatment on the number of extravasated (parenchymal) neutrophils in SAH animals. Neutrophil extravasation is reduced by PDTC. E: Number of collagenase-positive profiles in treated SAH animals, given as % of values in untreated SAH animals. All three treatments reduce the extent of vascular collagenase activity. F: Effect of PDTC treatment on post-SAH intravascular tracer leakage. Values are area fractions of intravascular (closed bars) and extravascular (open bars) FITC-albumin deposits. G: Representative images of striatum showing vascular collagenase activity in untreated and anti PMN treated animals sacrificed at 1 hour after SAH. Arrows: collagen IV stained vessels (red) positive for collagenase activity (green). Data are mean ± sem. N = 5 animals per treatment group. * Significantly different at p < 0.05 from untreated SAH animals.

Back to article page