Figure 1

PPCSE induces HO-1 protein and activity in bEnd.3 cells. A: Cells were treated with various concentrations of PPCSE for 24 h. B: Cells were treated with 30 μg/ml PPCSE for the indicated time intervals. C: Cells were pretreated with actinomycin D (Act.D) or cycloheximide (CHX) for 1 h, and then incubated with PPCSE for 24 h. A, B, C: The expression of HO-1 was determined by Western blot analysis. D: Cells were treated with various concentrations of PPCSE for 24 h, and then the activity of HO-1 was determined. Data are expressed as the mean ± SEM of three individual experiments. A, B, D: ^# P < 0.05 as compared with the cells exposed to vehicle alone. C: ^# P < 0.05 as compared with the cells exposed to PPCSE alone.