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Figure 4 | Journal of Neuroinflammation

Figure 4

From: The role of tumor necrosis factor-α and TNF-α receptors in cerebral arteries following cerebral ischemia in rat

Figure 4

Immunofluorescence staining for TNF-α; a) in the fresh, organ culture after 24 h incubation, organ culture after 48 h incubation, 24 h culture + treatment with IMD-0354, 48 h culture + treatment with IMD-0354, 24 h culture + treatment with SB386023-b, 48 h culture + treatment with SB386023-b, 24 h culture+ treatment with U0126 and 48 h culture + treatment with U0126. There was a clear increase in TNF-α protein level in the smooth muscle cell layer after 24 and 48 h culture as compared to fresh vessels. All three inhibitors significantly prevented the enhanced expression of TNF-α protein level in culture for 24 and 48 h. b) Bar graph demonstrating the fluorescence intensity for TNF-α. The results are expressed as mean values ± S.E.M. *P < 0.05 and **P < 0.01, significant difference between fresh and culture after 24 and 48 h. §P < 0.05 and §§P < 0.01, significant difference between 48 h organ culture and treatment with 48 h culture + (IMD-0354, SB386023-b and U0126). #P < 0.05 and ##P < 0.01, significant difference between 24 h organ culture and treatment with 24 h culture + (IMD-0354, SB386023-b and U0126).

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