Figure 2

TIMP-3 expression in astrocytes and OLs in ischemic EC at 72 h in Timp-3 WT mice. A. Photomicrographs show double immunofluorescent-labeling of TIMP-3 and GFAP in sham-operated and ischemic EC at 24 and 72 h of reperfusion. TIMP-3 was not expressed in GFAP-labeled astrocytes at 24 h, but by 72 h TIMP-3 was markedly expressed and co-localized with astrocytes (arrows). The co-localization of TIMP-3 and GFAP was confirmed by Z-stack confocal image. B. CC1-positive OLs express TIMP-3 (arrows) in ischemic EC at 72 h. The co-localization of TIMP-3 and CC1 was confirmed by Z-stack confocal image. Scale bars = 50 μm.