Intact axon, normal Wallerian degeneration, and kinetics of myelin clearance and Galectin-3/MAC-2 expression during normal Wallerian degeneration. (A) A Schwann cell that is surrounded by basal lamina (arrow heads) forms a myelin sheath around an intact axon; Bar 1 μm. (B) Axons are not detected 7 days after the injury, and Schwann cells (S) and a macrophage (m), which are situated within basal lamina sheaths (dark arrow heads), contain myelin fragments and lipid droplets in their cytoplasm (white arrow heads) (after ); Bar 2 μm. (C) Time course of myelin phagocytosis and degradation (Po) and Galectin-3/MAC-2 protein (Gal-3) production. Phagocytosis and degradation of myelin result in the reduction of tissue content of the myelin specific molecule Po. Nerve segments located 5 millimeters distal to lesion sites were removed from wild-type mice at the indicated times and used to determine tissue levels of Po and Gal-3 by ELISA. Those are presented as percentage of their maximal values that are defined 100% (after ).