NgR expression in human immune cells. (A) Expression of NgR1 mRNA in a panel of human immune cells, as determined by TaqMan RT qPCR. Expression relative to human foetal brain is depicted. Tex: ex vivo T cells; T cont: T cells cultured with anti-CD3 antibody for 2 days; T PHA-: T cells cultured with anti-CD3 and without PHA; T PHA+: T cells cultured with anti-CD3 and stimulated with PHA; BCL: B cell line; Mono ex: ex vivo monocytes; mono 7d: monocytes maintained in serum-free medium for 7 days; mono 7d IFN: 7d mono's treated with interferon gamma for the last 2 days; mono 7d LPS: 7d mono's treated with lipopolysaccharide for the last 2 days; Brain: commercially available human foetal brain RNA. (B) Expression of cell surface markers on human monocyte-derived DCs, quantified with flow cytometry. Bars represent mean of percentage of positive cells for the indicated marker, with SEM of 8 experiments, each representing a different donor. *P < 0.05, **P < 0.01, ***P < 0.001. (C) A representative western blot of NgR1 protein expression in human brain, immature and mature DC. (D) Relative mRNA expression of NgR1, NgR2, LINGO-1, TROY and p75NTR were determined with TaqMan RT qPCR. Mean values relative to immDC with SEM from 8 donors are shown. Wilcoxon matched-pairs signed rank test was used with delta Ct values to determine significance. *P < 0.05, **P < 0.01.