Figure 8

Sinomenine does not confer protection against oligomeric Aβ-induced direct toxicity to HT22 cells. A. An MTT reduction assay shows that ADDL treatment of HT22 cells reduced cell viability. The decrease in cell viability was not affected by sinomenine (n = 6 in all groups). There was no significant difference between ADDL and ADDL + sinomenine groups. B. TUNEL assay shows that sinomenine does not prevent ADDL-induced direct toxicity to HT22 cells. Sample images (B1) and quantified summary data (B2) of DAPI- or TUNEL-stained HT22 cells treated with different reagents show that ADDL treatment significantly increased the number of TUNEL-positive cells. The increase in the number of TUNEL-positive cells was not affected by sinomenine treatment (n = 3 in all groups). There was no significant difference between ADDL and ADDL + sinomenine groups. Scale bar, 100 μm. Asterisks denote significant differences (p < 0.05).