Figure 4

Activated microglial cells in the central avascular zone at P17. These cells are found in the superficial layer (s) of the central avascular zone (see cryosections). Activated microglial cells express GFP (green) under the control of the Cx3cr1 promoter and are additionally positive for lectin (red). Their morphology had changed from ramified cells to cells with short and broad processes (see flat mounts). E and F are the same as C and D, respectively, with the red channel omitted.