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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Pro-inflammatory cytokines and lipopolysaccharide induce changes in cell morphology, and upregulation of ERK1/2, iNOS and sPLA2-IIA expression in astrocytes and microglia

Figure 5

Cytokines or LPS + IFNγ induce NO production in different glial cell types. (A and B) BV-2 cells were serum starved in phenol red free DMEM for 4 h prior to treatment with the three-cytokine mixture (A) or LPS + IFNγ(B). At the respective time points, culture media were collected for determination of NO using the Greiss reaction protocol as described in the text. Data are mean ± SD from three independent experiments. **p < 0.01 vs. values at 12 h. One-way ANOVA, Dunnett's multiple comparison tests. (C) Comparing NO production between BV-2 microglial cells and rat primary microglial cells (RPM) based on protein in the dish. Results on RPM preparation have been repeated.

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Journal of Neuroinflammation

ISSN: 1742-2094