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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Pro-inflammatory cytokines and lipopolysaccharide induce changes in cell morphology, and upregulation of ERK1/2, iNOS and sPLA2-IIA expression in astrocytes and microglia

Figure 6

Cytokines induce sPLA2-IIA mRNA and protein expression in DITNC, and primary astrocytes. After exposing DITNC astrocytes to TNFα, IL-1β, IFNγ, and LPS for 24 h, sPLA2-IIA mRNA expression was determined by RT-PCR (A), and protein expression was determined by Western blot (C). Quantitative data are expressed as relative density to G3PDH (sPLA2-IIA vs. G3PDH) (B) or to β-actin (sPLA2-IIA vs. β-actin) (D). Results are mean ± SD from three independent experiments (*p < 0.05, **p < 0.01 vs. control, One-way ANOVA, Dunnett's multiple comparison test). (E) Only the three cytokines mixture could cause induction of sPLA2-IIA in primary astrocytes.

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