Figure 2

Change in mRNA expression in cortical explants over time. Time-dependent change in CCL4 and antisecretory factor (ASF) mRNA expression in SJL/J cortical explants. RNA was isolated from resected cortical tissue after incubation in explant medium for 0, 0.5, 2, and 4.5 hours. Transcript levels were referenced to β-actin mRNA levels. Values were expressed as fold difference relative to freshly isolated cortex (baseline) + SEM. Pooled cDNA from SJL/J mice (n = 4) were measured in replicates of four. * = P < 0.05 and ** = P < 0.01, relative to 0 hours, Dunnett's multiple comparison test.