Activation of p38 and p44/42 (ERK1/2) MAPKs in response to viral infection of primary microglia. A) Control uninfected (C) or virus-infected (+HSV) microglial cell culture lysates were collected at the indicated time points to assess MAPK activation using Western Blot. B) The kinetics of p38 MAPK activation were quantified in microglial cell cultures infected with HSV-1 using a FACE™ p38 Chemi, in-cell Western assay (Active Motif, Carlsbad, CA). Data presented are representative of mean ± SD with 3 replicates from 2 separate experiments. *p < 0.05 and** p < 0.01 vs. uninfected control.