Figure 1From: Lipocalin 2 in the central nervous system host response to systemic lipopolysaccharide administration Lcn2 and 24p3R mRNA levels in the brain and liver following intraperitoneal injection of LPS. Mice were injected i.p. with sterile 0.15 M NaCl with or without LPS (50 μg) and euthanized after 4 h or 24 h. An extra group of mice received a second LPS injection (dual) 16 h later, and were euthanized 4 h after the second injection. Total RNA was extracted from the brain (A) and the liver (B) and 5 μg analyzed by RPA as described in the Materials and Methods. Quantification of the Lcn2 and 24p3R RNA levels by densitometry (C). For statistical analysis an unpaired, two-tailed t-test was used. For statistical significance * p < 0.05 for LPS- versus vehicle-treated control mice (p < 0.05).Back to article page