Lcn2 and 24p3R mRNA levels in the brain and liver following intraperitoneal injection of LPS. Mice were injected i.p. with sterile 0.15 M NaCl with or without LPS (50 μg) and euthanized after 4 h or 24 h. An extra group of mice received a second LPS injection (dual) 16 h later, and were euthanized 4 h after the second injection. Total RNA was extracted from the brain (A) and the liver (B) and 5 μg analyzed by RPA as described in the Materials and Methods. Quantification of the Lcn2 and 24p3R RNA levels by densitometry (C). For statistical analysis an unpaired, two-tailed t-test was used. For statistical significance * p < 0.05 for LPS- versus vehicle-treated control mice (p < 0.05).