Lcn2 protein levels in the brain following intraperitoneal injection of LPS. Mice were treated as shown. Total protein was extracted from the brain and 50 μg analyzed by Western blot (A) as described in the Materials and Methods with quantification (B) of the Lcn2 protein relative to GAPDH loading control. Comparison of Lcn2 protein level by Western blot (C) in brain from WT versus Lcn2 KO mice after dual LPS-treated mice. For statistical significance *, p < 0.05 for LPS-treated mice versus control mice (p ≤ 0.05).