Regulation of Lcn2 mRNA levels in C8 microglial cells. The C8 microglial cell line was grown to 75% confluency and treated for 4 h at 37°C as indicted. Total RNA was isolated and analyzed by RPA as described in the Materials and Methods. For statistical analysis one-way ANOVA with Dunnett's multiple comparison test was used. For statistical significance * p < 0.05 versus untreated control (Con).