The level of various host response, chemokine and cytokine mRNA transcripts in the brain of WT versus Lcn2 KO mice following intraperitoneal injection of LPS. Mice were injected i.p. with sterile PBS with or without LPS and received a second LPS injection (dual) 16 h later and euthanized 4 h after the second injection. Total RNA was extracted from the brain and 10 μg analyzed by RPA as described in the Materials and Methods. For quantification of the mRNA levels, densitometry was performed on film autoradiographs and corrected for loading by reference to the L32 loading control. For statistical analysis, one-way ANOVA with Tukey's multiple comparison test was used. For statistical significance *, p < 0.05 for LPS-treated mice versus vehicle-injected control mice (p ≤ 0.05).