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Figure 12 | Journal of Neuroinflammation

Figure 12

From: Up-regulation of dorsal root ganglia BDNF and trkB receptor in inflammatory pain: an in vivo and in vitrostudy

Figure 12

Apoptosis analyses after acute BDNF treatment after TNF-α treatment. DRG cultures were pre-treated with 5 nM TNF-α for 48 hours followed by 400 ng/ml BDNF for 10 or 40 min. Cells were immediately fixed and apoptotic cells were detected by TUNEL reaction kit. (A) Control group; (B) TNF-α-pretreated group. DNase I-treated cells served as a positive control, while reaction enzyme removal served as a negative control. TUNEL staining was detected with a fluorescent green dye and cells were counterstained with DAPI. Insert illustrates an apoptotic cell from the positive control. Scale bars, 50 μm.

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