Basal and drug-evoked CGRP and substance P release in DRG cultures. After a 5 nM TNF-α treatment for 48 hours, 400 ng/ml BDNF was added for 30 or 60 min. The supernatants were collected and subjected to CGRP and substance P EIA analysis. Data are expressed as mean ± SEM, presented as percentage of control. Data from BDNF treatment were analyzed using one-way AVOVA followed by Tukey test, *p < 0.05, ***p < 0.001; compared corresponding basal groups. The comparison between TNF-α and control groups was analyzed using an un-paired Student's t-test, ##
p < 0.01, ###
p < 0.001 (N = 5~7 per group).