Figure 2From: Lipopolysaccharide modulates astrocytic S100B secretion: a study in cerebrospinal fluid and astrocyte cultures from rats S100B secretion is modified by LPS in astrocyte cultures and acute hippocampal slices. Rat cortical astrocytes were cultured in DMEM containing 10% FCS. After confluence, the medium was replaced by DMEM without serum in the presence or absence of LPS (from 0.01 to 30 μg/mL). S100B was measured by ELISA at 1 h (A) and 24 h (B). Each value is a mean (± standard error) of at least 5 independent experiments performed in triplicate. Means indicated by different letters are significantly different, assuming p < 0.05. (C) Adult Wistar rats were killed by decapitation and 0.3 mm hippocampal slices were obtained using a McIlwain chopper. After a metabolic recovery period, hippocampal slices were exposed to LPS (from 0.1 to 10 μg/mL) and the extracellular content of S100B measured by ELISA at 1 h. Each value is the mean (± standard error) of at least 5 independent experiments performed in triplicate. Means indicated by different letters are significantly different (one way ANOVA followed by Duncan's test, with a significance level of p < 0.05).Back to article page