Intracellular GFAP content is modified by LPS without change in intracellular S100B content in astrocytes. Rat cortical astrocytes were cultured in DMEM containing 10% FCS. After confluence, the medium was replaced by DMEM without serum in the presence or absence of LPS (from 0.01 to 30 μg/mL). Cells were lysed and intracellular contents of S100B (A) and GFAP (B) were measured by ELISA. Each value is the mean (± standard error) of at least 5 independent experiments performed in triplicate. Means indicated by different letters are significantly different (one way ANOVA followed by Duncan's test, with a significance level of p < 0.05).