Figure 1

Dopamine neurons in the adult female C57BL/6J mouse substantia nigra express Nox2, p47 ^phox and p67 ^phox subunits of the NADPH oxidase. Immunofluorescent histochemical staining revealed the presence of Nox2, p47^phoxand p67^phox(green) in the neurons positive for TH (red). Merged images indicate co-labeling (yellow). Occasionally cells positive for a subunit were not dopaminergic neurons (TH^-cells). To illustrate such an example, arrows in the top row point to a Nox2⁺/TH^- cell and arrowheads in the middle row indicate a cell that is p47^phox+/TH^-. Scale bars equal 20 μm.