Dopamine neurons in the adult female C57BL/6J mouse substantia nigra express Nox2, p47
phox and p67
phox subunits of the NADPH oxidase. Immunofluorescent histochemical staining revealed the presence of Nox2, p47phoxand p67phox(green) in the neurons positive for TH (red). Merged images indicate co-labeling (yellow). Occasionally cells positive for a subunit were not dopaminergic neurons (TH-cells). To illustrate such an example, arrows in the top row point to a Nox2+/TH- cell and arrowheads in the middle row indicate a cell that is p47phox+/TH-. Scale bars equal 20 μm.