Number, intensity and protein expression in SphK1 positive cells after LPS and DMS treatments. Quantitative analysis shows that the number of SphK1-positive AMC increased in cultures treated with LPS when compared to that in untreated cultures (A). This increase in number was not evident in cultures treated with DMS alone or DMS+LPS. Quantitative analysis reveals that the intensity of SphK1 immunofluorescence is increased in cultures treated with LPS when compared to that of untreated cultures (B). Western blot analysis of SphK1 protein levels in the corpus callosum of rats 1 h after LPS or DMS+LPS injection, and corresponding saline controls has been carried out (C, D). C, SphK1 (43kDa) and β-actin (43kDa) immunoreactive bands are shown. D, The optical density of SphK1 following LPS treatment is significantly increased when compared with the corresponding controls. Note that concomitant injection of DMS with LPS did not increase SphK1 protein levels (** p < 0.01, ΔΔ p < 0.01). All experiments were carried out in triplicate.