OX-42 (Aa, Ba, Ca) and TNF-α (Ab, Bb, Cb) expression in 5-d-old rats receiving saline, LPS or DMS + LPS injection and sacrificed 1 h later. The intensity of TNF-α immunoexpression in AMC is evidently augmented after LPS injection (Bb) when compared with that in the saline group (Ab) (arrows). This is more evident in merged images in Bc in comparison with Ac. In rats treated with DMS + LPS (Ca-c), TNF-α expression appears to be significantly reduced as shown by its co-localization with OX-42 in AMC (Ca-c). Nuclei are stained with DAPI. Scale bar = 50 μm.