Figure 4

Quantitative analysis of number, intensity and mRNA expression of SphK1 positive AMC. The number of SphK1-positive AMC (co-expressing OX-42) is increased 1 h after LPS injection (A). The intensity of SphK1 immunofluorescence is also increased significantly at 30 min to 1 h compared with that 6 h after LPS injection and that of control (B). SphK1 mRNA expression (C) is detected in primary microglial cells in vitro and its expression was induced significantly in cells exposed to LPS for 1 h, as revealed by RT-PCR (D) The data are normalized with the housekeeping gene, β-actin (C) and presented as mean ± S.E. Control vs LPS, * P < 0.05, ** P < 0.01.,*** P < 0.001