Levels of SphK1 mRNA expression and immunofluorescence intensity and the number of SphK1 positive cells are reduced after LPS and DMS treatments. RT-PCR analysis demonstrates that SphK1 mRNA expression is increased more than 25 fold in corpus callosum of rats injected with LPS, and the concomitant injection of DMS prevented this increase of SphK1 (A). Injection of DMS alone did not have any effect on SphK1 mRNA expression. Quantitative analysis shows that the number of SphK1-positive AMC decreased in rats injected with DMS+LPS, when compared to that in rats injected with LPS alone (B), Further, the intensity of SphK1 immunofluorescence was also decreased in rats injected with DMS+LPS when compared to that in rats injected with LPS alone (C). Data are presented as mean ± S.E. * vs control, * P < 0.05, ** P < 0.01, *** P < 0.001 Scale bar = 50 μm.